Coding
Part:BBa_M36996
Designed by: Nathan Barnett, Meghan Bowler, Kian Torabian Group: Stanford BIOE44 - S11 (2011-04-28)
Non-Glycolated Antifreeze Protein (AFP) - 10-Repeated Segment
The standard non-glycolated antifreeze protein (AFP) from the Pseudopleuronecta americanus. Begins with a chain of C's and is followed by 10 rough repeats of "ANAAAAAALTA". The codons have been optimized for E. coli.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 32
Illegal PstI site found at 98
Illegal PstI site found at 170
Illegal PstI site found at 182
Illegal PstI site found at 281
Illegal PstI site found at 329 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 32
Illegal PstI site found at 98
Illegal PstI site found at 170
Illegal PstI site found at 182
Illegal PstI site found at 281
Illegal PstI site found at 329
Illegal NotI site found at 133
Illegal NotI site found at 232 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 360
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 32
Illegal PstI site found at 98
Illegal PstI site found at 170
Illegal PstI site found at 182
Illegal PstI site found at 281
Illegal PstI site found at 329 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 32
Illegal PstI site found at 98
Illegal PstI site found at 170
Illegal PstI site found at 182
Illegal PstI site found at 281
Illegal PstI site found at 329 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//collections/antifreeze
protein | Antifreeze Protein |